Departmental seminar

A dose-response in a cell model

Does compound X reduce inflammatory signalling, and at what concentration?

Presenter Name, Lab of Cell SignallingFictional study, sample deckOctober 2026

Compound X lowers IL-6 release in a dose-dependent way, with no loss of viability.

Methods

Four steps, three replicates

  1. Culture

    Macrophage-like cells, passage 6 to 9.

  2. Dose

    0, 1, 5, 25 micromolar, 2 hours.

  3. Stimulate

    LPS for 24 hours.

  4. Measure

    IL-6 by ELISA, viability by MTT.

IL-6 falls as the dose rises

Half the control level at 25 micromolar.

DoseIL-6
0 uM840
1 uM790
5 uM610
25 uM420
Mean of three experiments, fictional data

The numbers behind the claim

3Independent experiments
50%IL-6 reduction at 25 uM
>95%Cell viability at every dose

Reading the result

Claim, evidence, caveat

  1. Compound X reduces IL-6 release.

  2. Monotonic fall across four doses; viability unchanged.

  3. One cell line, one stimulus, no in vivo data yet.

  4. Repeat in primary cells

    Then test a second inflammatory readout.

Thank you

Questions

Presenter Presenter Namelab@example.edu